abstract：:During dengue outbreaks, acute diagnosis at the patient's point of need followed by appropriate supportive therapy reduces morbidity and mortality. To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of d...

journal_title：Diagnostic microbiology and infectious disease

authors： Dauner AL,Mitra I,Gilliland T Jr,Seales S,Pal S,Yang SC,Guevara C,Chen JH,Liu YC,Kochel TJ,Wu SJ

更新日期：2015-09-01 00:00:00

abstract：:We evaluated the performance of the 3 automated systems (Cepheid Xpert, BD MAX, and IMDx C. difficile for Abbott m2000) detecting Clostridium difficile toxin gene compared to toxigenic culture. Of the 254 stool specimens tested, 87 (48 slight, 35 moderate, and 4 heavy growth) were toxigenic culture positive. The overa...

journal_title：Diagnostic microbiology and infectious disease

authors： Yoo J,Lee H,Park KG,Lee GD,Park YG,Park YJ

更新日期：2015-09-01 00:00:00

abstract：:Rhinocladiella, a genus of black yeast-like fungi, is related to many infections in humans, including not only mild cutaneous lesions but also fatal brain infections. However, endophthalmitis caused by Rhinocladiella has never been reported by far. Herein, we present the first case of endophthalmitis due to Rhinocladi...

journal_title：Diagnostic microbiology and infectious disease

authors： Liu M,Xin X,Li J,Chen S

更新日期：2015-09-01 00:00:00

abstract：:Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nuc...

journal_title：Diagnostic microbiology and infectious disease

authors： Edwards T,Burke P,Smalley HB,Gillies L,Longhurst D,Vipond B,Hobbs G

更新日期：2015-09-01 00:00:00

abstract：:In Brazil, since 2009, there has been an ever increasing widespread of the bla(KPC-2) gene, mainly in Klebsiella pneumoniae. This study aims to assess the molecular epidemiology and genetic background of this gene in Enterobacteriaceae (non-K. pneumoniae) species from 9 Brazilian states between 2009 and 2011. Three hu...

journal_title：Diagnostic microbiology and infectious disease

authors： Tavares CP,Pereira PS,Marques Ede A,Faria C Jr,de Souza Mda P,de Almeida R,Alves Cde F,Asensi MD,Carvalho-Assef AP

更新日期：2015-08-01 00:00:00

abstract：:The Study for Monitoring Antimicrobial Resistance Trends has monitored the in vitro activity of several recommended antimicrobials used in the management of intra-abdominal infections (IAIs) globally since 2002. In this report, we document the changing susceptibility patterns to recommended antimicrobials in Klebsiell...

journal_title：Diagnostic microbiology and infectious disease

authors： Kazmierczak KM,Lob SH,Hoban DJ,Hackel MA,Badal RE,Bouchillon SK

更新日期：2015-07-01 00:00:00

abstract：:Early diagnosis of leptospirosis in humans is critical with regard to initiation of appropriate treatment; however, the gold standard serological test cannot detect antibodies until nearly a week after symptom onset. PCR has been shown to be sensitive and specific in the early phase of leptospirosis. Previously, we de...

journal_title：Diagnostic microbiology and infectious disease

authors： Galloway RL,Hoffmaster AR

更新日期：2015-07-01 00:00:00

abstract：:Antituberculosis (TB) chemotherapeutic drugs may cause a variety of adverse drug reactions (ADRs). To assess the potential of drug-induced lymphocyte stimulation test (DLST) in screening ADRs in patients treated with anti-TB drugs, we performed DLST in 272 TB patients (176 cases with ADRs and 96 controls without ADRs)...

journal_title：Diagnostic microbiology and infectious disease

authors： Sun Q,Sha W,Gui XW,Xiao YJ,Zeng WH,Sun WW,Xiao HP,Ye WY

更新日期：2015-06-01 00:00:00

abstract：:Predicted and observed failures at higher cefepime MICs have prompted the Clinical and Laboratories Standards Institute (CLSI) to lower the susceptible breakpoint for Enterobacteriaceae to ≤2mg/L, with dose-dependent susceptibility at 4-8mg/L, while the susceptibility breakpoint for nonfermentative organisms remain un...

journal_title：Diagnostic microbiology and infectious disease

authors： Rhodes NJ,Liu J,McLaughlin MM,Qi C,Scheetz MH

更新日期：2015-06-01 00:00:00

abstract：:Avibactam is a novel β-lactamase inhibitor that restores the activity of otherwise hydrolyzed β-lactams against Gram-negative bacteria expressing different classes of serine β-lactamases. In the last decade, β-lactam-avibactam combinations were tested against a variety of clinical isolates expressing multiple commonly...

journal_title：Diagnostic microbiology and infectious disease

authors： Papp-Wallace KM,Bajaksouzian S,Abdelhamed AM,Foster AN,Winkler ML,Gatta JA,Nichols WW,Testa R,Bonomo RA,Jacobs MR

更新日期：2015-05-01 00:00:00

abstract：:The aim of the study was to evaluate the influence of dietary and demographic factors and some habits on the prevalence of Helicobacter pylori infection in 150 dyspeptic patients examined endoscopically and by the urea breath test. Positivity rate was lower (50.6%) in patients consuming honey ≥1 day weekly compared wi...

journal_title：Diagnostic microbiology and infectious disease

authors： Boyanova L,Ilieva J,Gergova G,Vladimirov B,Nikolov R,Mitov I

更新日期：2015-05-01 00:00:00

abstract：:Coxiella burnetii, a Gram-negative intracellular bacterium, can give rise to Q fever in humans and is transmitted mainly by inhalation of infected aerosols from animal reservoirs. Serology is commonly used to diagnose Q fever, but the early cellular immune response-i.e., C. burnetii-specific interferon γ (IFN-γ) produ...

journal_title：Diagnostic microbiology and infectious disease

authors： Schoffelen T,Self JS,Fitzpatrick KA,Netea MG,van Deuren M,Joosten LA,Kersh GJ

更新日期：2015-04-01 00:00:00

abstract：:The 2013 LEADER surveillance program monitored the in vitro activity of linezolid and comparator agents against Gram-positive bacteria at 60 medical centers in the United States. A total of 7183 pathogens were contributed from 6 predetermined pathogen groups. The groups were Staphylococcus aureus, coagulase-negative s...

journal_title：Diagnostic microbiology and infectious disease

authors： Flamm RK,Mendes RE,Hogan PA,Ross JE,Farrell DJ,Jones RN

更新日期：2015-04-01 00:00:00

abstract：:Typing of bacterial isolates has been used for decades to study local outbreaks as well as in national and international surveillances for monitoring newly emerging resistant clones. Despite being recognized as a nosocomial pathogen, the precise modes of transmission of Stenotrophomonas maltophilia in health care sett...

journal_title：Diagnostic microbiology and infectious disease

authors： Gherardi G,Creti R,Pompilio A,Di Bonaventura G

更新日期：2015-03-01 00:00:00

abstract：:Ceftaroline fosamil was approved in 2010 by the United States Food and Drug Administration (USA-FDA) for the treatment of patients with acute bacterial skin and skin structure infections (ABSSSIs) and community-acquired bacterial pneumonia (CABP). After approval, several studies and case reports have described the pos...

journal_title：Diagnostic microbiology and infectious disease

authors： Stryjewski ME,Jones RN,Corey GR

更新日期：2015-03-01 00:00:00

abstract：:The first cases of infective endocarditis due to a new species of Bergeyella, Bergeyella cardium sp. nov., are reported. Two strains, 13-07(T) (= JCM 30115(T) = NCCP 15908(T)) and 13-16, were independently isolated from 2 patients in different hospitals in Korea. Initially, the isolates were identified as Brevundimona...

journal_title：Diagnostic microbiology and infectious disease

authors： Sohn KM,Huh K,Baek JY,Kim YS,Kang CI,Peck KR,Lee NY,Song JH,Ko KS,Chung DR

更新日期：2015-03-01 00:00:00

abstract：:In this study, we present population-based data regarding the prevalence of aerococci in clinical urinary samples. During a 3-month period, all aerococcal isolates from urinary samples from 2 clinical microbiology laboratories were collected. We identified 64 Aerococcus urinae isolates and 40 Aerococcus sanguinicola i...

journal_title：Diagnostic microbiology and infectious disease

authors： Senneby E,Petersson AC,Rasmussen M

更新日期：2015-02-01 00:00:00

abstract：:Cupriavidus bacteremia is a rare infection and identification of the pathogen is difficult. We present four cases of bacteremia by Cupriavidus metallidurans that were initially identified to the genus level by both Bruker and Vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry and later ...

journal_title：Diagnostic microbiology and infectious disease

authors： D'Inzeo T,Santangelo R,Fiori B,De Angelis G,Conte V,Giaquinto A,Palucci I,Scoppettuolo G,Di Florio V,Giani T,Sanguinetti M,Rossolini GM,Spanu T

更新日期：2015-01-01 00:00:00

abstract：:The Verigene Gram-positive blood culture assay (BC-GP) is a microarray-based rapid diagnostic test, which includes targets for 12 bacterial species and 3 resistance determinants. We prospectively compared the diagnostic accuracy of the BC-GP to routine microbiologic methods and evaluated the potential of the BC-GP for...

journal_title：Diagnostic microbiology and infectious disease

authors： Aitken SL,Hemmige VS,Koo HL,Vuong NN,Lasco TM,Garey KW

更新日期：2015-01-01 00:00:00

abstract：:We compared the performances and the cost-effectiveness of 5 selective media for Group B Streptococcus (GBS) screening in vaginal samples from pregnant women. The usefulness of these media is unquestionable for GBS screening; the choice will depend largely on the laboratory organization. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Joubrel C,Gendron N,Dmytruk N,Touak G,Verlaguet M,Poyart C,Réglier-Poupet H

更新日期：2014-12-01 00:00:00

abstract：:This pilot study evaluates the diagnostic performance of Sofia RSV Fluorescent Immunoassay Analyzer (FIA) and Sofia Influenza A + B FIA for rapid detection of respiratory syncytial virus and influenza A and B. Sofia had a lower-than-expected sensitivity for all viruses and a high rate of false-positive results for inf...

journal_title：Diagnostic microbiology and infectious disease

authors： Bruning AH,van Dijk K,van Eijk HW,Koen G,van Woensel JB,Kruisinga FH,Pajkrt D,Wolthers KC

更新日期：2014-12-01 00:00:00

abstract：:The cerebrospinal fluid (CSF) polymerase chain reaction (PCR) is the gold standard to detect cerebral viral activity. As positive findings do not prove an impact on the neurological disorder, data interpretation is difficult. To better assess the impact of positive CSF PCR findings in different neurological diseases a...

journal_title：Diagnostic microbiology and infectious disease

authors： Kleines M,Scheithauer S,Schiefer J,Häusler M

更新日期：2014-11-01 00:00:00

abstract：:Salmonella serotyping is an essential first step for identification of isolates associated with disease outbreaks. The Salmonella genoserotyping array (SGSA) is a microarray-based alternative to standard serotyping designed to rapidly identify 57 of the most commonly reported serovars through detection of the genes en...

journal_title：Diagnostic microbiology and infectious disease

authors： Yoshida C,Lingohr EJ,Trognitz F,MacLaren N,Rosano A,Murphy SA,Villegas A,Polt M,Franklin K,Kostic T,OIE Reference Laboratory for Salmonellosis.,Austrian Agency for Health and Food Safety.,Kropinski AM,Card RM

更新日期：2014-11-01 00:00:00

abstract：:The incidence of carbapenem-resistant Pseudomonas aeruginosa (CRPA) bacteremia has increased in recent years, and infections caused by CRPA result in higher mortality than those caused by susceptible strains. This study was performed to evaluate the risk factors for mortality and to study the impact of virulence facto...

journal_title：Diagnostic microbiology and infectious disease

authors： Jeong SJ,Yoon SS,Bae IK,Jeong SH,Kim JM,Lee K

更新日期：2014-10-01 00:00:00

abstract：:We aimed to analyze the efficacy and safety of high doses of daptomycin (10 mg/kg/d) plus rifampin (D10 + R) for prosthetic joint infection (PJI). This was an observational retrospective multicenter study (2010-2012) including all patients with acute PJI by fluoroquinolone-resistant staphylococci managed with implant ...

journal_title：Diagnostic microbiology and infectious disease

authors： Lora-Tamayo J,Parra-Ruiz J,Rodríguez-Pardo D,Barberán J,Ribera A,Tornero E,Pigrau C,Mensa J,Ariza J,Soriano A

更新日期：2014-09-01 00:00:00

abstract：:Using quantitative PCR (qPCR), we differentiated asymptomatic and symptomatic Indian Leishmania donovani infection. qPCR on blood of 40 visceral leishmaniasis, 130 endemic, and 40 non-endemic healthy controls showed 500 times less (P < .0001) parasitemia in asymptomatic compared to the symptomatic ones and threshold o...

journal_title：Diagnostic microbiology and infectious disease

authors： Sudarshan M,Sundar S

更新日期：2014-09-01 00:00:00

abstract：:Treatment options for multidrug-resistant pathogens remain problematic in many regions and individual countries, warranting ongoing surveillance and analysis. Limited antimicrobial susceptibility information is available for pathogens from Vietnam. This study determined the bacterial susceptibility of aerobic gram-neg...

journal_title：Diagnostic microbiology and infectious disease

authors： Biedenbach DJ,Bouchillon SK,Hoban DJ,Hackel M,Phuong DM,Nga TT,Phuong NT,Phuong TT,Badal RE

更新日期：2014-08-01 00:00:00

abstract：:Streptococcus pneumoniae (pneumococcus) is the most common bacterial etiology of community-acquired pneumonia (CAP) in adults, a leading cause of death. The majority of pneumococcal CAP is diagnosed by blood culture, which likely underestimates the burden of disease. The 2007 CAP guidelines recommend routine use of th...

journal_title：Diagnostic microbiology and infectious disease

authors： Harris AM,Beekmann SE,Polgreen PM,Moore MR

更新日期：2014-08-01 00:00:00

abstract：:The frequency of superantigen production among Staphylococcus aureus isolates associated with endocarditis is not well defined. We tested 154 S. aureus isolates from definite infective endocarditis cases for the presence of staphylococcal enterotoxins A-E, H, and TSST-1 by PCR, enzyme-linked immunosorbent assay, and u...

journal_title：Diagnostic microbiology and infectious disease

authors： Chung JW,Karau MJ,Greenwood-Quaintance KE,Ballard AD,Tilahun A,Khaleghi SR,David CS,Patel R,Rajagopalan G

更新日期：2014-06-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a tool for identifying clinically relevant anaerobes. We evaluated the analytical performance characteristics of the Bruker Microflex with Biotyper 3.0 software system for identification of anaerobes and examined...

journal_title：Diagnostic microbiology and infectious disease

authors： Hsu YM,Burnham CA

更新日期：2014-06-01 00:00:00

abstract：:Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, ...

journal_title：Diagnostic microbiology and infectious disease

authors： Hardick J,Sadiq S,Perelstein E,Peterson S,Rothman R,Gaydos CA

更新日期：2014-06-01 00:00:00

abstract：:This work comprises 9 pulmonary nontuberculous mycobateria isolates obtained from sputum of 4 different patients from Brazil. The sequencing and phylogenetic analysis allowed their accurate identification as Mycobacterium intracellulare. We report a mutation at position 453 creating a new HaeIII cutting site and, ther...

journal_title：Diagnostic microbiology and infectious disease

authors： Caldas PC,Campos CE,Dos Reis LM,Ferreira NV,de Carvalho LD,da Silva MV,Medeiros RF,Montes FC,Ramos JP

更新日期：2014-06-01 00:00:00

abstract：:A nested polymerase chain reaction (PCR) targeting the 56-kDa antigen gene is currently the most commonly used molecular technique for confirmation of scrub typhus and genotyping of Orientia tsutsugamushi. In this study, we have compared the commonly used nested PCR (N-PCR) with a single-step conventional PCR (C-PCR) ...

journal_title：Diagnostic microbiology and infectious disease

authors： Janardhanan J,Prakash JA,Abraham OC,Varghese GM

更新日期：2014-05-01 00:00:00

abstract：:Complicated skin and skin structure infections (cSSSIs) and intra-abdominal infections (IAIs) are problematic due to decreasing therapeutic options available against multidrug-resistant pathogens common among these types of infections. A total of 2245 isolates from African and the Middle Eastern (AfME) countries were ...

journal_title：Diagnostic microbiology and infectious disease

authors： Renteria MI,Biedenbach DJ,Bouchillon SK,Hoban DJ,Raghubir N,Sajben P,Mokaddas E

更新日期：2014-05-01 00:00:00

abstract：:This study evaluates matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) capability for the identification of difficult-to-identify microorganisms. A total of 150 bacterial isolates inconclusively identified with conventional phenotypic tests were further assessed by 16S rRNA se...

journal_title：Diagnostic microbiology and infectious disease

authors： Rodríguez-Sánchez B,Marín M,Sánchez-Carrillo C,Cercenado E,Ruiz A,Rodríguez-Créixems M,Bouza E

更新日期：2014-05-01 00:00:00

abstract：:This study examined the serotype distribution and antimicrobial resistance of pneumococcal isolates from invasive infections in children between 2006 and 2010, when the 7-valent pneumococcal conjugate vaccine (PCV7) was offered as an optional vaccine in Korea. Among 140 isolates collected from 8 centers, the common se...

journal_title：Diagnostic microbiology and infectious disease

authors： Cho EY,Lee H,Choi EH,Kim YJ,Eun BW,Cho YK,Kim YK,Jo DS,Lee HS,Lee J,Kim MN,Kim DS,Lee HJ

更新日期：2014-04-01 00:00:00

abstract：:The standard culture for identification of Mycobacterium tuberculosis takes a long time to perform. We introduce here a method for fast identification of M. tuberculosis in mycobacterial culture system. Antibodies to Antigen (Ag) 85 of M. tuberculosis were produced and subsequently used to develop enzyme-linked immuno...

journal_title：Diagnostic microbiology and infectious disease

authors： Phunpae P,Chanwong S,Tayapiwatana C,Apiratmateekul N,Makeudom A,Kasinrerk W

更新日期：2014-03-01 00:00:00

abstract：:Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis reveale...

journal_title：Diagnostic microbiology and infectious disease

authors： Iraz M,Duzgun AO,Cicek AC,Bonnin RA,Ceylan A,Saral A,Nordmann P,Sandalli C

更新日期：2014-03-01 00:00:00

abstract：:The present study was conducted to investigate the prevalence and diversity of Arcobacter and Campylobacter spp. in 298 stool samples of patients with diarrhoea, collected from 22 Portuguese hospitals, between September and November 2012. Detection of Arcobacter and Campylobacter spp. was performed using molecular-bas...

journal_title：Diagnostic microbiology and infectious disease

authors： Ferreira S,Júlio C,Queiroz JA,Domingues FC,Oleastro M

更新日期：2014-03-01 00:00:00

abstract：:A new real-time reverse transcription polymerase chain reaction (RT-PCR) assay, the AccuPower Norovirus Real-time RT-PCR Kit, was evaluated in detection of human norovirus in stool specimens. Studies for detection limit, dynamic range, reproducibility, and cross-reactivity were performed. A total of 281 fecal specimen...

journal_title：Diagnostic microbiology and infectious disease

authors： Hyun J,Kim HS,Kim HS,Lee KM

更新日期：2014-01-01 00:00:00